Dr. Wei Qiang (Candidate for the Macromolecular Assemblies position)
Laboratory of Chemical Physics, NIDDK National Institute
Thursday, December 8, 2011 - 11:00 to 12:00
1116-1118 Marcus Nanotechnology Bldg
Application of Solid State NMR to Macromolecular Assemblies and Membrane Proteins
Solid state nuclear magnetic resonance (NMR) spectroscopy is an advanced high-resolution technique which provides substantial structural information for the non-crystalline and insoluble systems such as macromolecular assemblies and membrane proteins. In this presentation, I am going to focus on two specific topics. (1) The first high resolution structural model for an antiparallel beta sheet amyloid fibril formed by full length beta amyloid peptide. I will show that using a novel generation seeding procedure, I can selectively capture a metastable intermediate state of amyloid fibril with predominant antiparallel beta sheet architecture. In addition, I utilized a number of solid state NMR techniques to identify the key structural features from local conformation to the assembly of individual beta amyloid peptide monomers. (2) Solid state NMR studies on the human immunodeficiency virus (HIV) membrane fusion mechanism. I will show that solid state NMR can provide molecular-level details about the membrane disruption induced by the insertion of the HIV fusion peptides. This information is crucial to understand the membrane fusion of HIV, which is the initial stage of the viral infection.